Visit us at our booth 4-E41 and take the chance for a personal conversation. These data suggest that RECQ5 removes RAD51 filaments stabilizing stalled replication forks at CFSs and hence facilitates CFS cleavage by MUS81-EME1. 'Microsynth is a European leader in offering services related to DNA/RNA synthesis, various kinds of DNA/RNA analyses and molecular biology project outsourcing. Microsynth France will be present at Forum Labo Paris 2023 in Paris/France on 28 - 30 March, 2023. Biochemically, RECQ5 alleviates the inhibitory effect of RAD51 on 3′-flap DNA cleavage by MUS81-EME1 through its RAD51 filament disruption activity. Gonzalez-Garcia Department Molecular Genetics Center for Research in Agricultural Genomics Barcelona, Spain Christoph R. This leads to defective chromosome segregation and accumulation of CFS-associated DNA damage in G1 cells. RECQ5 depletion or mutational inactivation of its ATP-binding site, RAD51-interacting domain, or phosphorylation site causes excessive binding of RAD51 to CFS loci and impairs CFS expression. The parent company has its headquarter in Balgach, Switzerland. Upon replication stress, RECQ5 associates with CFSs in early mitosis through its physical interaction with MUS81 and promotes MUS81-dependent mitotic DNA synthesis. Microsynth Seqlab is the German subsidiary of Microsynth AG and is located in Gttingen. Here, we show that these DNA transactions are promoted by RECQ5 DNA helicase in a manner dependent on its Ser727 phosphorylation by CDK1. The MUS81-EME1 endonuclease cleaves late replication intermediates at common fragile sites (CFSs) during early mitosis to trigger DNA-repair synthesis that ensures faithful chromosome segregation.
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